Koji Kinoshita

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+45 65 504 578
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Dr. Kinoshita joined in Single Particle Science and Engineering, SPSE, group of department of physics, chemistry and pharmacy at SDU in January of 2013.

Before he joined in the group, he was performing single molecule force spectroscopy experiment, which was combined with micropipette manipulation technique, at University of British Columbia in Vancouver of Canada for more than 10 years. The unique biophysical experiment technique, which is called Biomembrane Force Probe method (BFP), was developed to study dynamic strength of molecular adhesion bonds between cells or the extracellular matrix in more than two orders of magnitude in force (10-12 – 10-9 N).

Micropipette manipulation technique is not only powerful tool to study cell biology in single molecular level, but also can use as bioengineering tool of micro and nano particle research. In the group of SPSE, he is targeting to study single particle for liposomal nanotechnology development and biomedical research, which can derive nano or micro pharmaceutical drug and fabrication of drug delivery device for cancer therapy. His skills and knowledge of cell adhesion measurement in single molecular level will connect those new biomaterial research fields of single particle in next step.

Education

  • 1996-1999: Ph.D. in Materials Science, Shizuoka University (Japan), Advisor: Prof. Masahito Yamazaki, "The effect of the interaction free energy between the segments of the membrane surface and solvents for the structure and stability of biomembrane"
  • 1994-1996: M.S. in Physics, Shizuoka University (Japan), Advisor: Prof. Masahito Yamazaki, "Dependence of c parameter for the structure and stability of biomembrane"

Professional Experience

  • 2013-Present: Associate Professor/Lab. Manager SPSE, Department of Molecular Medicine, SDU, Odense (DENMARK), 

  • 2009-2012: Research Associate Department of Pathology and Laboratory of Medicine, University of British Columbia, Vancouver, BC (CANADA)

  • 2004-2008: Senior Research Associate Department of Biomedical Engineering, University of Boston, Boston, MA (USA)

  • 2001-2004: Research Assistant Physics and Astronomy, University of British Columbia, Vancouver, BC (CANADA)

  • 2000-2001: Research Assistant Biochemistry, McMaster University, Hamilton, ON (CANADA)

  • 1999-2000: Part-Time Research Fellow Satellite Venture Business Laboratory, Shizuoka University, Shizuoka (JAPAN)

Publications

  1. Koji Kinoshita, Elisa Parra, and David Needham, "Adsorption of ionic surfactants at microscopic air-water interfaces using the Micropipette interfacial area-expansion method: Measurement of the diffusion coefficient and renormalization of the mean ionic activity for SDS ", J. Colloid and Interface Science, (2017),  https://doi.org/10.1016/j.jcis.2017.05.077
  2. Koji Kinoshita, Elisa Parra, and David Needham, “New Sensitive Micro-Measurements of Dynamic Surface Tension and Diffusion Coefficients: Validated and Tested for the Adsorption of 1-Octanol at a Microscopic Air-Water Interface and its Dissolution into Water”,  J. Colloid and Interface Science, 488, 166-179 (2017)

  3. Koji Kinoshita, Elisa Parra, Abdirazak Hussein, Anders Utoft, Prasad Walke, Robin de Bruijn and David Needham, “From Single Microparticles to Microfluidic Emulsification: Fundamental Properties (Solubility, Density, Phase Separation) from Micropipette Manipulation of Solvent, Drug and Polymer Microspheres”, Processes, 4, 49; doi:10.3390/pr4040049 (2016)

  4. Elisa Parra, Koji Kinoshita and David Needham, “Micropipette technique study of natural and synthetic lung surfactants at the air-water interface: Presence of a sp-b analog peptide promotes membrane aggregation, formation of tightly stacked lamellae, and growth of myelin figures.”, Langmuir, 32, 10570-10581 (2016)

  5. David Needham, Amina Arslanagic, Kasper Glud, Pablo Hervella, Leena Karimi, Poul Flemming Høilund-Carlsen, Koji Kinoshita, Jan Mollenhauer, Elisa Parra, Anders Utoft, and Prasad Walke, “Bottom Up Design of Nanoparticles (to eventually treat cancer): “Put the drug in the Cancer’s food”, J. Drug Targeting, DOI: 10.1080/1061186X.2016.1238092 (2016)

  6. Joachim Habel, Anayo Ogbonna, Nanna Larsen, Sol`ene Cherr´e, Søren Kynde, Søren Roi Midtgaard, Koji Kinoshita, Simon Krabbe, Grethe Vestergaard Jensen, Jesper Søndergaard Hansen, Kristoffer Almdal and Claus H`elix-Nielsen, “Selecting analytical tools for characterization of polymersomes in aqueous solution”, RSC Adv., 5, 79924-79946 (2015) 

  7. Kinoshita, K., Leung, A., Simon, S., and Evans E. Long-Lived, High-Strength States of ICAM-1 Bonds to b2 Integrin, II: Lifetimes of Bonds to Recombinant aLb2 Under Force, Biophys. J. 98, 1467-1475 (2010)

  8. Evans, E., Kinoshita, K., Simon, S., and Leung, A. Long-Lived, High-Strength States of ICAM-1 Bonds to b2 Integrin, I: Lifetimes of Bonds to Recombinant aLb2 Under Force, Biophys. J. 98, 1458-1466 (2010)

  9. ​Kinoshita, K., Leung, A., and Evans, E. Direct Force Measurement of Cell Adhesion Molecules with Biomembrane Force Probe (BFP) Method, Seibutsu Butsuri, 49, 30-33 (2009)

  10. Evans, E., Halvorsen, K., Kinoshita, K., and Wong, W. A New Approach to Analysis of Single-Molecule Force Measurements, Hinterdorfer, P., eds., in Handbook of Single Molecule Biophysics, 571-589 (2009)

  11.  Evans, E. and Kinoshita, K. Using Force to Probe Single-Molecule Receptor-Cytoskeletal Anchoring beneath the Surface of a Living Cell Methods in Cell Biology, Wang and Discher, eds., Methods in Cell Biology. Vol. 83, 373-396 (2007)

  12. Evans, E., Heinrich, V., Leung, A., and Kinoshita, K Nano- to Microscale Dynamics of P-Selectin Detachment from Leukocyte Interface: I. Membrane Separation from the Cytoskeleton, Biophys. J. 88, 2288-2298 (2005)

  13. Tanaka, T., Li, S., Kinoshita, K., and Yamazaki, M. La3+ stabilizes the hexagonal II (HII) phase in phosphatidylethanolamine membranes, Biochim. Biophys. Acta, 1515, 189-201 (2001)

  14. Kinoshita, K., Li, S., and Yamazaki, M. The mechanism of the stabilization of the hexagonal II (HII) phase in phosphatidylethanolamine membranes in the presence of low concentrations of dimethysulfoxide., Eur. Biophys. J. 30, 207-220 (2001)

  15. Yamashita, Y., Kinoshita, K., and Yamazaki, M. Low concentration of DMSO stabilizes the bilayer gel phase rather than the interdigitated gel phase in dihexadecylphosphatidylcholine membranes. Biochim. Biophys. Acta, 1467, 395-405 (2000)

  16. Li, S., Kinoshita, K., Furuike, S., and Yamazaki, M. Effects of solvents interacting favorably with hydrophilic segments of the membrane surface of phosphatidylcholine on their gel-phase membranes in water. Biophys. Chem. 81, 191-196 (1999)

  17. Kinoshita, K., Furuike, S., and Yamazaki, M. Intermembrane distance in multilamellar vesicles of phosphatidylcholine depends on the interaction free energy between solvents and the hydrophilic segments of the membrane surface. Biophys. Chem. 74, 237-249 (1998)

  18. Kinoshita, K., and Yamazaki, M. Phase transition between hexagonal II (HII) and liquid-crystalline phase induced by interaction between solvents and segments of the membrane surface of dioleoylphosphatidylcholine. Biochim. Biophys. Acta, 1330, 199-206 (1997)

  19. Hatanaka, Y., Kinoshita, K., and Yamazaki M. Osmotic stress induces a phase transition from interdigitated gel phase to bilayer gel phase in multilamellar vesicles of dihexadecylphosphatidylcholine. Biophys. Chem.65, 229-233 (1997)

  20. Kinoshita, K., Asano, T., and Yamazaki, M., Interaction of the surface of biomembrane with solvents: Structure of multilamellar vesicles of dipalmitoylphosphatidylcholine in acetone-water mixtures. Chem. Phys. Lipids, 85, 53-65. (1997)

  21. Kinoshita, K., and Yamazaki, M. Organic solvents induce interdigitated gel structures in multilamellar vesicles of dipalmitoylphosphatidylcholine. Biochim. Biophys. Acta, 1284, 233-239 (1996)